![asuragen/AmplideX® PCR/CE C9orf72 Kit/50/49581](images/no_picture.gif)
AmplideX® PCR/CE C9orf72 Kit
The AmplideX PCR/CE C9orf72 Kit (RUO) is a research product for the detection of GGGGCC repeats in the C9orf72 gene. These reagents provide a single-tube PCR approach based on a Repeat-Primed PCR (RP-PCR) design to profile repeat sequences in the C9orf72 gene.
Features & Benefits
Asuragen has established the market-leading technology for the reliable amplification and analysis of CGG triplet repeats in the FMR1 gene. The AmplideX PCR/CE C9orf72 Kit (RUO) is an expansion of this technology into the neurogenetics space. The kit provides clinical and pharmaceutical researchers with a reliable and reproducible method for high-resolution genotyping of hexanucleotide repeat expansions in the C9orf72 gene, which is of increasing interest in frontotemporal dementia (FTD) – the second most common form of early onset dementia after Alzheimer’s Disease – and amyotrophic lateral sclerosis (ALS).
Reduced ComplexityAnalysis of the C9orf72 gene has been simplified through:
- Implementation of proprietary 2-in-1 PCR solution for amplifying GC-rich regions
- A single sourced kit consisting of all PCR reagents needed for C9orf72 repeat amplification
- Streamlined workflow with minimal hands-on time
Optimized WorkflowValuable operator hands-on time has been significantly reduced through:
- Single PCR reaction for both sizing and screening
- Direct injection of PCR products (no PCR clean up) to Capillary Electrophoresis platforms
- Decreased need for Southern blot analysis
Quality PerformancePerforming C9orf72 analysis with greater sensitivity and accuracy:
- Five-fold improvement in length of alleles sized: accurate sizing up to 145 repeats
- Detection of alleles greater than 145 repeats
- Reveal low-level mosaicism and minor alleles
*For Research Use Only. Not for use in Diagnostic procedures.
Analytical Characteristics
Analytical characteristics of AmplideX PCR/CE C9orf72 Kit:
- Accurately sizes up to 145 repeats (Figure 1)
- Detects alleles >145 repeats (Figure 2)
- Resolves zygosity (Figure 3)
Figure 1: Coriell cell line ND06769: The AmplideX Kit correctly sizes a primary allele at 13 repeats and a minor allele at 44 repeats. The expanded allele is also detected in the electropherogram.
Figure 2. Coriell cell line ND06769: Alleles up to 145 repeats can be detected above background and correctly sized, alleles above 145 repeats are amplified and detected as expanded.
Figure 3: Side by side comparison of 2 Coriell cell lines: ND08619 (7,7 homozygous) and ND12102 (7,expanded heterozygous): Extension of the repeat-primed peak pattern beyond the shorter allele clearly differentiates the heterozygous sample from homozygous sample.
Ordering
Product Name | Number of Reactions | Catalog Number |
---|---|---|
AmplideX® PCR/CE C9orf72 Kit (RUO) | 50 | 49581 |
T 1-877-777-1874; 512-681-5200 F 512-681-5202 E orders@asuragen.com
AmplideX® PCR/CE C9orf72 Kit
The AmplideX PCR/CE C9orf72 Kit* is a research product for the detection of GGGGCC repeats in the C9orf72 gene. These reagents provide a single-tube PCR approach based on a Repeat-Primed PCR (RP-PCR) design to profile repeat sequences in the C9orf72 gene.
Features & Benefits
Asuragen has established the market-leading technology for the reliable amplification and analysis of CGG triplet repeats in the FMR1 gene. The AmplideX PCR/CE C9orf72 Kit (RUO) is an expansion of this technology into the neurogenetics space. The kit provides clinical and pharmaceutical researchers with a reliable and reproducible method for high-resolution genotyping of hexanucleotide repeat expansions in the C9orf72 gene, which is of increasing interest in frontotemporal dementia (FTD) – the second most common form of early onset dementia after Alzheimer’s Disease – and amyotrophic lateral sclerosis (ALS).
Reduced ComplexityAnalysis of the C9orf72 gene has been simplified through:
- Implementation of proprietary 2-in-1 PCR solution for amplifying GC-rich regions
- A single sourced kit consisting of all PCR reagents needed for C9orf72 repeat amplification
- Streamlined workflow with minimal hands-on time
Optimized WorkflowValuable operator hands-on time has been significantly reduced through:
- Single PCR reaction for both sizing and screening
- Direct injection of PCR products (no PCR clean up) to Capillary Electrophoresis platforms
- Decreased need for Southern blot analysis
Quality PerformancePerforming C9orf72 analysis with greater sensitivity and accuracy:
- Five-fold improvement in length of alleles sized: accurate sizing up to 145 repeats
- Detection of alleles greater than 145 repeats
- Reveal low-level mosaicism and minor alleles
Analytical Characteristics
Analytical characteristics of AmplideX PCR/CE C9orf72 Kit:
- Accurately sizes up to 145 repeats (Figure 1)
- Detects alleles >145 repeats (Figure 2)
- Resolves zygosity (Figure 3)
Figure 1: Coriell cell line ND06769: The AmplideX Kit correctly sizes a primary allele at 13 repeats and a minor allele at 44 repeats. The expanded allele is also detected in the electropherogram.
Figure 2. Coriell cell line ND06769: Alleles up to 145 repeats can be detected above background and correctly sized, alleles above 145 repeats are amplified and detected as expanded.
Figure 3: Side by side comparison of 2 Coriell cell lines: ND08619 (7,7 homozygous) and ND12102 (7,expanded heterozygous): Extension of the repeat-primed peak pattern beyond the shorter allele clearly differentiates the heterozygous sample from homozygous sample.
Ordering
Product Name | Number of Reactions | Catalog Number |
---|---|---|
AmplideX® PCR/CE C9orf72 Kit (RUO) | 50 | 49581 |
T 1-877-777-1874; 512-681-5200 F 1-512-681-5202 E orders@asuragen.com
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多谢……
没有序列识别的功能,他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应,需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接,也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶(Reversetranscripatase)是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA(cDNA)的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。
2. 禽成髓细胞瘤病毒(AMV)反转录酶:有强的聚合酶活性和RNA酶H活性。最适作用温度为42℃。
3.Thermus thermophilus、Thermus flavus等嗜热微生物的热稳定性反转录酶:在Mn存在下,允许高温反转录RNA,以消除RNA模板的二级结构。
4.MMLV反转录酶的RNase H突变体:商品名为SuperScript 和SuperScriptⅡ。此种酶较其它酶能多将更大部分的RNA转换成cDNA,这一特性允许从含二级结构的、低温反转录很困难的mRNA模板合成较长cDNA。向左转|向右转
而且保真性要高一点的,因为今后要用来做表达
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